Nucleolar localization of non-structural protein 3b, a protein specifically encoded by the severe acute respiratory syndrome coronavirus.
Identifieur interne : 004736 ( Main/Exploration ); précédent : 004735; suivant : 004737Nucleolar localization of non-structural protein 3b, a protein specifically encoded by the severe acute respiratory syndrome coronavirus.
Auteurs : Xiaoling Yuan [République populaire de Chine] ; Zhenyu Yao ; Yajun Shan ; Bo Chen ; Zhen Yang ; Jie Wu ; Zhenhu Zhao ; Jiapei Chen ; Yuwen CongSource :
- Virus research [ 0168-1702 ] ; 2005.
Descripteurs français
- KwdFr :
- Animaux, Cellules COS, Cellules Vero, Humains, Lignée cellulaire, Nucléole (métabolisme), Phosphoprotéines (métabolisme), Protéines chromosomiques nonhistones (métabolisme), Protéines de liaison à l'ARN (métabolisme), Protéines nucléaires (métabolisme), Protéines virales non structurales (), Protéines virales non structurales (génétique), Protéines virales non structurales (métabolisme), Protéines à fluorescence verte (génétique), Protéines à fluorescence verte (métabolisme), Signaux de triage des protéines, Séquence d'acides aminés, Transfection, Virus du SRAS (génétique), Virus du SRAS (métabolisme), Virus du SRAS (pathogénicité).
- MESH :
- génétique : Protéines virales non structurales, Protéines à fluorescence verte, Virus du SRAS.
- métabolisme : Nucléole, Phosphoprotéines, Protéines chromosomiques nonhistones, Protéines de liaison à l'ARN, Protéines nucléaires, Protéines virales non structurales, Protéines à fluorescence verte, Virus du SRAS.
- pathogénicité : Virus du SRAS.
- Animaux, Cellules COS, Cellules Vero, Humains, Lignée cellulaire, Protéines virales non structurales, Signaux de triage des protéines, Séquence d'acides aminés, Transfection.
English descriptors
- KwdEn :
- Amino Acid Sequence, Animals, COS Cells, Cell Line, Cell Nucleolus (metabolism), Chlorocebus aethiops, Chromosomal Proteins, Non-Histone (metabolism), Green Fluorescent Proteins (genetics), Green Fluorescent Proteins (metabolism), Humans, Nuclear Proteins (metabolism), Phosphoproteins (metabolism), Protein Sorting Signals, RNA-Binding Proteins (metabolism), SARS Virus (genetics), SARS Virus (metabolism), SARS Virus (pathogenicity), Transfection, Vero Cells, Viral Nonstructural Proteins (chemistry), Viral Nonstructural Proteins (genetics), Viral Nonstructural Proteins (metabolism).
- MESH :
- chemical , chemistry : Viral Nonstructural Proteins.
- chemical , genetics : Green Fluorescent Proteins, Viral Nonstructural Proteins.
- chemical , metabolism : Chromosomal Proteins, Non-Histone, Green Fluorescent Proteins, Nuclear Proteins, Phosphoproteins, RNA-Binding Proteins, Viral Nonstructural Proteins.
- genetics : SARS Virus.
- metabolism : Cell Nucleolus, SARS Virus.
- pathogenicity : SARS Virus.
- Amino Acid Sequence, Animals, COS Cells, Cell Line, Chlorocebus aethiops, Humans, Protein Sorting Signals, Transfection, Vero Cells.
Abstract
The open reading frame 3 (ORF3) of the severe acute respiratory syndrome coronavirus (SARS-CoV) genome encodes a predicted 154-amino acid protein, which lacks similarities to any known protein, and is named 3b. In this study, it was shown that 3b protein was predominately localized to nucleus with EGFP tag at its N- or C-terminus. The localization patterns were similar in different transfected cells. Immuno-fluorescence assay revealed that 3b protein was co-localized well with C23 in nucleolus. C23, B23 and fibrillarin all are important nucleolar proteins, which localize in the region of the nucleolus. Co-transfection of p3b-EGFP with pC23-DsRed, pB23-DsRed and pfibrillarin-DsRed further confirmed 3b's nucleolus localization. With construction of serial truncated mutants of 3b, a region (residues 134-154 aa) responsible for nucleolar localization was determinated in 3b protein. These results provide a new insight for further functional studies of SARS-CoV 3b protein.
DOI: 10.1016/j.virusres.2005.06.001
PubMed: 16046244
Affiliations:
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uniqKey="Shan Y" first="Yajun" last="Shan">Yajun Shan</name></author><author><name sortKey="Chen, Bo" sort="Chen, Bo" uniqKey="Chen B" first="Bo" last="Chen">Bo Chen</name></author><author><name sortKey="Yang, Zhen" sort="Yang, Zhen" uniqKey="Yang Z" first="Zhen" last="Yang">Zhen Yang</name></author><author><name sortKey="Wu, Jie" sort="Wu, Jie" uniqKey="Wu J" first="Jie" last="Wu">Jie Wu</name></author><author><name sortKey="Zhao, Zhenhu" sort="Zhao, Zhenhu" uniqKey="Zhao Z" first="Zhenhu" last="Zhao">Zhenhu Zhao</name></author><author><name sortKey="Chen, Jiapei" sort="Chen, Jiapei" uniqKey="Chen J" first="Jiapei" last="Chen">Jiapei Chen</name></author><author><name sortKey="Cong, Yuwen" sort="Cong, Yuwen" uniqKey="Cong Y" first="Yuwen" last="Cong">Yuwen Cong</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2005">2005</date><idno type="RBID">pubmed:16046244</idno><idno type="pmid">16046244</idno><idno 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a protein specifically encoded by the severe acute respiratory syndrome coronavirus.</title><author><name sortKey="Yuan, Xiaoling" sort="Yuan, Xiaoling" uniqKey="Yuan X" first="Xiaoling" last="Yuan">Xiaoling Yuan</name><affiliation wicri:level="1"><nlm:affiliation>Department of Pathophysiology, Beijing Institute of Radiation Medicine, No. 27 Taiping Road, Beijing 100850, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Pathophysiology, Beijing Institute of Radiation Medicine, No. 27 Taiping Road, Beijing 100850</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Yao, Zhenyu" sort="Yao, Zhenyu" uniqKey="Yao Z" first="Zhenyu" last="Yao">Zhenyu Yao</name></author><author><name sortKey="Shan, Yajun" sort="Shan, Yajun" uniqKey="Shan Y" first="Yajun" last="Shan">Yajun Shan</name></author><author><name sortKey="Chen, Bo" sort="Chen, Bo" uniqKey="Chen B" first="Bo" last="Chen">Bo Chen</name></author><author><name sortKey="Yang, Zhen" sort="Yang, Zhen" uniqKey="Yang Z" first="Zhen" last="Yang">Zhen Yang</name></author><author><name sortKey="Wu, Jie" sort="Wu, Jie" uniqKey="Wu J" first="Jie" last="Wu">Jie Wu</name></author><author><name sortKey="Zhao, Zhenhu" sort="Zhao, Zhenhu" uniqKey="Zhao Z" first="Zhenhu" last="Zhao">Zhenhu Zhao</name></author><author><name sortKey="Chen, Jiapei" sort="Chen, Jiapei" uniqKey="Chen J" first="Jiapei" last="Chen">Jiapei Chen</name></author><author><name sortKey="Cong, Yuwen" sort="Cong, Yuwen" uniqKey="Cong Y" first="Yuwen" last="Cong">Yuwen Cong</name></author></analytic><series><title level="j">Virus research</title><idno type="ISSN">0168-1702</idno><imprint><date when="2005" type="published">2005</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Amino Acid Sequence</term><term>Animals</term><term>COS Cells</term><term>Cell Line</term><term>Cell Nucleolus (metabolism)</term><term>Chlorocebus aethiops</term><term>Chromosomal Proteins, Non-Histone (metabolism)</term><term>Green Fluorescent Proteins (genetics)</term><term>Green Fluorescent Proteins (metabolism)</term><term>Humans</term><term>Nuclear Proteins (metabolism)</term><term>Phosphoproteins (metabolism)</term><term>Protein Sorting Signals</term><term>RNA-Binding Proteins (metabolism)</term><term>SARS Virus (genetics)</term><term>SARS Virus (metabolism)</term><term>SARS Virus (pathogenicity)</term><term>Transfection</term><term>Vero Cells</term><term>Viral Nonstructural Proteins (chemistry)</term><term>Viral Nonstructural Proteins (genetics)</term><term>Viral Nonstructural Proteins (metabolism)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Animaux</term><term>Cellules COS</term><term>Cellules Vero</term><term>Humains</term><term>Lignée cellulaire</term><term>Nucléole (métabolisme)</term><term>Phosphoprotéines (métabolisme)</term><term>Protéines chromosomiques nonhistones (métabolisme)</term><term>Protéines de liaison à l'ARN (métabolisme)</term><term>Protéines nucléaires (métabolisme)</term><term>Protéines virales non structurales ()</term><term>Protéines virales non structurales (génétique)</term><term>Protéines virales non structurales (métabolisme)</term><term>Protéines à fluorescence verte (génétique)</term><term>Protéines à fluorescence verte (métabolisme)</term><term>Signaux de triage des protéines</term><term>Séquence d'acides aminés</term><term>Transfection</term><term>Virus du SRAS (génétique)</term><term>Virus du SRAS (métabolisme)</term><term>Virus du SRAS (pathogénicité)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Viral Nonstructural Proteins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Green Fluorescent Proteins</term><term>Viral Nonstructural Proteins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Chromosomal Proteins, Non-Histone</term><term>Green Fluorescent Proteins</term><term>Nuclear Proteins</term><term>Phosphoproteins</term><term>RNA-Binding Proteins</term><term>Viral Nonstructural Proteins</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>SARS Virus</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Protéines virales non structurales</term><term>Protéines à fluorescence verte</term><term>Virus du SRAS</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Cell Nucleolus</term><term>SARS Virus</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Nucléole</term><term>Phosphoprotéines</term><term>Protéines chromosomiques nonhistones</term><term>Protéines de liaison à l'ARN</term><term>Protéines nucléaires</term><term>Protéines virales non structurales</term><term>Protéines à fluorescence verte</term><term>Virus du SRAS</term></keywords><keywords scheme="MESH" qualifier="pathogenicity" xml:lang="en"><term>SARS Virus</term></keywords><keywords scheme="MESH" qualifier="pathogénicité" xml:lang="fr"><term>Virus du SRAS</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Amino Acid Sequence</term><term>Animals</term><term>COS Cells</term><term>Cell Line</term><term>Chlorocebus aethiops</term><term>Humans</term><term>Protein Sorting Signals</term><term>Transfection</term><term>Vero Cells</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Animaux</term><term>Cellules COS</term><term>Cellules Vero</term><term>Humains</term><term>Lignée cellulaire</term><term>Protéines virales non structurales</term><term>Signaux de triage des protéines</term><term>Séquence d'acides aminés</term><term>Transfection</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The open reading frame 3 (ORF3) of the severe acute respiratory syndrome coronavirus (SARS-CoV) genome encodes a predicted 154-amino acid protein, which lacks similarities to any known protein, and is named 3b. In this study, it was shown that 3b protein was predominately localized to nucleus with EGFP tag at its N- or C-terminus. The localization patterns were similar in different transfected cells. Immuno-fluorescence assay revealed that 3b protein was co-localized well with C23 in nucleolus. C23, B23 and fibrillarin all are important nucleolar proteins, which localize in the region of the nucleolus. Co-transfection of p3b-EGFP with pC23-DsRed, pB23-DsRed and pfibrillarin-DsRed further confirmed 3b's nucleolus localization. With construction of serial truncated mutants of 3b, a region (residues 134-154 aa) responsible for nucleolar localization was determinated in 3b protein. These results provide a new insight for further functional studies of SARS-CoV 3b protein.</div></front></TEI><affiliations><list><country><li>République populaire de Chine</li></country><settlement><li>Pékin</li></settlement></list><tree><noCountry><name sortKey="Chen, Bo" sort="Chen, Bo" uniqKey="Chen B" first="Bo" last="Chen">Bo Chen</name><name sortKey="Chen, Jiapei" sort="Chen, Jiapei" uniqKey="Chen J" first="Jiapei" last="Chen">Jiapei Chen</name><name sortKey="Cong, Yuwen" sort="Cong, Yuwen" uniqKey="Cong Y" first="Yuwen" last="Cong">Yuwen Cong</name><name sortKey="Shan, Yajun" sort="Shan, Yajun" uniqKey="Shan Y" first="Yajun" last="Shan">Yajun Shan</name><name sortKey="Wu, Jie" sort="Wu, Jie" uniqKey="Wu J" first="Jie" last="Wu">Jie Wu</name><name sortKey="Yang, Zhen" sort="Yang, Zhen" uniqKey="Yang Z" first="Zhen" last="Yang">Zhen Yang</name><name sortKey="Yao, Zhenyu" sort="Yao, Zhenyu" uniqKey="Yao Z" first="Zhenyu" last="Yao">Zhenyu Yao</name><name sortKey="Zhao, Zhenhu" sort="Zhao, Zhenhu" uniqKey="Zhao Z" first="Zhenhu" last="Zhao">Zhenhu Zhao</name></noCountry><country name="République populaire de Chine"><noRegion><name sortKey="Yuan, Xiaoling" sort="Yuan, Xiaoling" uniqKey="Yuan X" first="Xiaoling" last="Yuan">Xiaoling Yuan</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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